Ludwig KR, Schroll MM, Hummon AB. Comparison of In-Solution, FASP, and S-Trap Based Digestion Methods for Bottom-Up Proteomic Studies. J. Proteome Res. 2018, 17 (7), pp 2480–2490.
HaileMariam M, Eguez RV, Singh H, Shiferaw B, Ameni G, Pieper R, Yu Y. S-Trap is an ultrafast sample preparation approach for shotgun proteomics. J. Proteome Res., Just Accepted; DOI: 10.1021/acs.jproteome.8b00505.
S-Traps yielded the highest reproducibility, highest number of proteins and peptides identified, and highest number of PSMs. Table from Yang, Y., Anderson, E., & Zhang, S. Evaluation of six sample preparation procedures for qualitative and quantitative proteomics analysis of milk fat globule membrane. Electrophoresis, April, 2018.