Protein preparation products will soon be available from the microgram to multiple milligram scale. Large scale is particularly useful for those doing pre-enrichment for example in phosphopeptide or SISCAPA/immunoaffinity analysis.
“I tested Tryp-N in a modified STrap, fast-digestion sample prep system. I am pleased with its performance. Tryp-N worked specifically and robustly in a 1 hr, 65°C digestion in a 20% acetonitrile/Tryp-N buffer. For cellular lysate digestion, Tryp-N provided similar output to trypsin both for numbers of identified proteins and peptide identification rates with the expected inversion of ion fragments.“ — Dr. Alexandre Zougman, Team Leader in Clinical Proteomics, University of Leeds.