“The S-Trap is by far the best way to digest whole cell lysates, especially those with SDS. The benefits include significantly reduced sample handling (no need to precipitate and struggle to solubilize again) as well as fast digestion times and instrument friendly output with little further clean-up needed.” –This is really a great product!
“I tested Tryp-N in a modified STrap, fast-digestion sample prep system. I am pleased with its performance. Tryp-N worked specifically and robustly in a 1 hr, 65°C digestion in a 20% acetonitrile/Tryp-N buffer. For cellular lysate digestion, Tryp-N provided similar output to trypsin both for numbers of identified proteins and peptide identification rates with the expected inversion of ion fragments.“ — Dr. Alexandre Zougman, Team Leader in Clinical Proteomics, University of Leeds.